Impact of Adenosine A2 Receptor Ligands on BCL2 Expression in Skeletal Muscle Cells

Background: Adenosine plays the role of regulating cell differentiation, proliferation, and apoptosis in various kinds cells through B-cell lymphoma 2 (BCL2) pathway. Objectives: Since anti-apoptotic expression a controlling some lines, this study was designed to investigate whether adenosine analogue, NECA (non-selective receptors agonist), selective A2B receptor antagonist, PSB 603, A2A agonist, CG21680, affect BCL2-gene skeletal muscle rats. The purpose investigation test hypothesis that CG21680 treatment would significantly intensify BCL2 gene rat muscle. Methods: Flasks measuring 25 cm2 were employed culturing L6 cells. After treating these differential cells, relative mRNA level for gene, at varying conditions treatment, measured using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Results: From qRT-PCR analysis results, it concluded markedly amplified after CGS21680 (p < 0.01) treatment. More prospective validation receptors’ contribution modulating by delivered outcomes from combined pre-treatment with 603. These show when starved are treated combination 100 nM there substantial decrease comparison either used on its own. Conclusions: This study’s results showed, first time, an increase within Hence, escalation protein might have protective play against avert damage